Protective Immunity in Mice Immunized With P. vivax MSP1₁₉-Based Formulations and Challenged With P. berghei Expressing PvMSP1₁₉.

ABSTRACT: The lack of continuous in vitro cultures has been an obstacle delaying pre-clinical testing of Plasmodium vivax vaccine formulations based on known antigens. In this study, we generated a model to test available formulations based on the P. vivax MSP1₁₉ antigen. The Plasmodium berghei strains ANKA and NK65 were modified to express PvMSP1₁₉ instead of the endogenous PbMSP1₁₉. The hybrid parasites were used to challenge C57BL/6 or BALB/c mice immunized with PvMSP1₁₉-based vaccine formulations. The PvMSP1₁₉ was correctly expressed in the P. berghei hybrid mutant lines as confirmed by immunofluorescence using anti-PvMSP1₁₉ monoclonal antibodies and by Western

SUBMITTER: Dobrescu I 

PROVIDER: S-EPMC7045055 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature