An Improved Methodology to Evaluate Cell and Molecular Signals in the Reparative Callus During Fracture Healing.

ABSTRACT: Approximately 5% to 10% of all bone fractures do not heal completely, contributing to significant patient suffering and medical costs. Even in healthy individuals, fracture healing is associated with significant downtime and loss of productivity. However, no pharmacological treatments are currently available to promote efficient bone healing. A better understanding of the underlying molecular mechanisms is crucial for developing novel therapies to hasten healing. The early reparative callus that forms around the site of bone injury is a fragile tissue consisting of shifting cell populations held together by loose connective tissue. The delicate callus is challenging to section and is vulnerable to disintegration during the harsh steps of immunostaining, namely, decalcification, deparaffini

SUBMITTER: Valiya Kambrath A 

PROVIDER: S-EPMC7045301 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature