Loci associated with variation in gene expression and growth in juvenile salmon are influenced by the presence of a growth hormone transgene.

ABSTRACT:

Background

Growth regulation is a complex process influenced by genetic and environmental factors. We examined differences between growth hormone (GH) transgenic (T) and non-transgenic (NT) coho salmon to elucidate whether the same loci were involved in controlling body size and gene expression phenotypes, and to assess whether physiological transformations occurring from GH transgenesis were under the influence of alternative pathways. The following genomic techniques were used to explore differences between size classes within and between transgenotypes (T vs. NT): RNA-Seq/Differentially Expressed Gene (DEG) analysis, quantitative PCR (qPCR) and OpenArray analysis, Genotyping-by-Sequencing, and Genome-Wide Association Study (GWAS).

Results

DEGs identified in comparisons be

SUBMITTER: McClelland EK 

PROVIDER: S-EPMC7045383 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature