The use of deuterium-labeled gamma-aminobutyric (D₆-GABA) to study uptake, translocation, and metabolism of exogenous GABA in plants.

ABSTRACT:

Background

Exogenous application of gamma-aminobutyric acid (GABA) could relieve stress symptoms caused by abiotic stresses including anoxia, heat and cold shock, drought, and salt. However, studying translocation and metabolism of exogenous GABA is challenged by the presence of endogenous GABA.

Results

Herein, we used D₆-GABA in order to investigate the uptake, translocation, and the metabolism of exogenous GABA in Mexican lime (Citrus aurantifolia) seedlings using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis showed that D₆-GABA could be easily distinguished from the non-labeled GABA after methyl chloroformate (MCF) derivatization. The D₆-GABA was detected in the cortex (phloem), inner stem (xylem), and leav

SUBMITTER: Hijaz F 

PROVIDER: S-EPMC7045438 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature