Protein-based biorefining driven by nitrogen-responsive transcriptional machinery.

ABSTRACT:

Background

Protein-based bioconversion has been demonstrated as a sustainable approach to produce higher alcohols and ammonia fertilizers. However, owing to the switchover from transcription mediated by the bacterial RNA polymerase σ⁷⁰ to that mediated by alternative σ factors, the biofuel production driven by σ⁷⁰-dependent promoters declines rapidly once cells enter the stationary phase or encounter stresses. To enhance biofuel production, in this study the growth phase-independent and nitrogen-responsive transcriptional machinery mediated by the σ⁵⁴ is exploited to drive robust protein-to-fuel conversion.

Results

We demonstrated that disrupting the Escherichia coli ammonia assimilation pathways driven by glutamate dehydrogenase and g

SUBMITTER: Ma L 

PROVIDER: S-EPMC7045595 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature