Fourier transform infrared spectroscopy of developing bone mineral: from amorphous precursor to mature crystal.

ABSTRACT: Bone mineral development has been described to proceed through an amorphous precursor prior to apatite crystallization. However, further analytical approaches are necessary to identify specific markers of amorphous mineral components in bone. Here, we establish an original Fourier transform infrared (FTIR) spectroscopy approach to allow the specific identification of the amorphous and/or crystalline nature of bone mineral. Using a series of standards, our results demonstrate that obtaining the second derivative of the FTIR spectra could reveal a peak specifically corresponding to amorphous calcium phosphate (ACP) at ∼992 cm-1. The intensity of this peak was strongly correlated to ACP content in standard mixtures. The analysis of a variety of bones showed that a clear ACP peak could be iden

SUBMITTER: Querido W 

PROVIDER: S-EPMC7046087 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature