Non-enzymatic reaction of carnosine and glyceraldehyde-3-phosphate accompanies metabolic changes of the pentose phosphate pathway.

ABSTRACT:

Objectives

Carnosine (β-alanyl-l-histidine) is a naturally occurring dipeptide that selectively inhibits cancer cell growth, possibly by influencing glucose metabolism. As its precise mode of action and its primary targets are unknown, we analysed carnosine's effect on metabolites and pathways in glioblastoma cells.

Materials and methods

Glioblastoma cells, U87, T98G and LN229, were treated with carnosine, and metabolites were analysed by gas chromatography coupled with mass spectrometry. Furthermore, mitochondrial ATP production was determined by extracellular flux analysis and reaction products of carnosine were investigated using mass spectrometry.

Results

Carnosine decreased the intracellular abundance of several metabolites indicating a reduced activity of the pe

SUBMITTER: Oppermann H 

PROVIDER: S-EPMC7046307 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature