YAP promotes the proliferation of neuroblastoma cells through decreasing the nuclear location of p27^Kip1 mediated by Akt.

ABSTRACT:

Objective

We aimed to investigate the roles and underlying mechanisms of YAP in the proliferation of neuroblastoma cells.

Methods

The expression level of YAP was evaluated by Western blotting and immunocytochemistry. Cell viability, cell proliferation and growth were detected by CCK-8, PH3 and Ki67 immunostaining, and the real-time cell analyser system. The nuclear and cytoplasmic proteins of p27^Kip1 were dissociated by the nuclear-cytosol extraction kit and were detected by Western blotting and immunocytochemistry. mRNA levels of Akt, CDK5 and CRM1 were determined by qRT-PCR.

Results

YAP was enriched in SH-SY5Y cells (a human neuroblastoma cell line). Knock-down of YAP in SH-SY5Y cells or SK-N-SH cell line (another human neuroblastoma cell line) significan

SUBMITTER: Shen X 

PROVIDER: S-EPMC7046475 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature