Retinal blood vessel-origin yes-associated protein (YAP) governs astrocytic maturation via leukaemia inhibitory factor (LIF).

ABSTRACT:

Objectives

To testify that endothelial cells (ECs) induce astrocyte maturation by leukaemia inhibitory factor (LIF) secretion.

Materials and methods

In vivo experiments, mice bearing floxed alleles of YAP were crossed with mice expressing a Cre recombinase driven by the endothelial Tek promoter (Tek-Cre) to finally obtain the following three genotypes: YAP^f/f , Tek-Cre; YAP^f/w , Tek-Cre; and YAP^f/f . Retinal vascularization and astrocyte network were evaluated by whole-mount fluorescence and Western blotting. In vitro, experiments were performed in an astrocyte and human microvascular endothelial cell (HMEC-1) coculture model to analyse the mechanisms underlying the effect of endothelial YAP on astrocytes.

Results

In vivo, YAP^f/f<

SUBMITTER: Ai LQ 

PROVIDER: S-EPMC7046482 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature