Lipidomic identification of plasma lipids associated with pain behaviour and pathology in a mouse model of osteoarthritis.

ABSTRACT:

Introduction

Osteoarthritis (OA) is the most common form of joint disease, causing pain and disability. Previous studies have demonstrated the role of lipid mediators in OA pathogenesis.

Objectives

To explore potential alterations in the plasma lipidomic profile in an established mouse model of OA, with a view to identification of potential biomarkers of pain and/or pathology.

Methods

Pain behaviour was assessed following destabilisation of the medial meniscus (DMM) model of OA (n = 8 mice) and compared to sham controls (n = 7). Plasma and knee joints were collected at 16 weeks post-surgery. Plasma samples were analysed using ultra-high performance liquid chromatography accurate mass high resolution mass spectrometry (UHPLC-HR-MS) to identify potential differences in

SUBMITTER: Pousinis P 

PROVIDER: S-EPMC7046574 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature