Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture.

ABSTRACT: The perfusion of medium through blood vessels allows the preservation of donor organs and culture of bioengineered organs. However, tissue damage due to inadequate perfusion remains a problem. We evaluated whether intermittent external pressurization would improve the perfusion and viability of organs in culture. A bioreactor system was used to perfuse and culture rat small intestine and femoral muscle preparations. Intermittent positive external pressure (10 mmHg) was applied for 20 s at intervals of 20 s. Intermittent pressurization resulted in uniform perfusion of small intestine preparations and minimal tissue damage after 20 h of perfusion, whereas non-pressurized (control) preparations exhibited significantly worse perfusion of the upper surface than the lower surface and histologic

SUBMITTER: Sano K 

PROVIDER: S-EPMC7046602 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature