Oligomerization and characteristics of phosphoenolpyruvate carboxylase in Synechococcus PCC 7002.

ABSTRACT: Phosphoenolpyruvate carboxylase (PEPc) is an essential enzyme in plants. A photosynthetic form is present both as dimer and tetramer in C4 and CAM metabolism. Additionally, non-photosynthetic PEPcs are also present. The single, non-photosynthetic PEPc of the unicellular cyanobacterium Synechococcus PCC 7002 (Synechococcus), involved in the TCA cycle, was examined. Using size exclusion chromatography (SEC) and small angle X-ray scattering (SAXS), we observed that PEPc in Synechococcus exists as both a dimer and a tetramer. This is the first demonstration of two different oligomerization states of a non-photosynthetic PEPc. High concentration of Mg²⁺, the substrate PEP and a combination of low concentration of Mg²⁺ and HCO₃^- induced the tetramer fo

SUBMITTER: Durall C 

PROVIDER: S-EPMC7046716 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature