Molecular epidemiology of Blastocystis spp. in children referred to Qods hospital in northwest of Iran.

ABSTRACT: Blastocystis sp. is a polymorphic intestinal parasite in humans and animals. The parasite has a worldwide distribution, especially in developing countries with poor sanitation, exposure to animals, and improper disposal systems. The aim of this study was to identify the subtypes of Blastocystis sp. among children of Qazvin, northwest Iran. Totally, 864 stool samples were collected from the children referred to Qods hospital in Qazvin, Iran. Fecal specimens were investigated by formalin-ethyl acetate concentration method and trichrome staining as well as cultivation of all samples in clotted fetal bovine medium. DNA extraction of culture-positive specimens and PCR amplification of 18S ribosomal RNA gene region was performed. The sequences detected were compared with reference

SUBMITTER: Niaraki SR 

PROVIDER: S-EPMC7046871 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature