Use of LUCS (Light-Up Cell System) as an alternative live cell method to predict human acute oral toxicity.

ABSTRACT: LUCS (Light-Up Cell System) is a new live cell test that allows assessment of a cell's homeostasis and its alteration by a toxic agent. To evaluate the effectiveness of LUCS as an alternative test method for acute oral toxicity, we compared EC₅₀s determined in HepG2 cells treated with 53 chemicals selected from the ACuteTox EU database with corresponding human blood LC₅₀s derived from human acute poisoning cases. Linear regression analysis showed that LUCS results predict human data to 69 %. Rodent oral LD₅₀s and LUCS EC₅₀s were then correlated to human LC₅₀s using shared data sets. Linear regression analyses comparing LUCS and animal data clearly showed that LUCS always predicts human toxicity better than animal data do. These success

SUBMITTER: Gironde C 

PROVIDER: S-EPMC7047139 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature