Single-cell transcriptomics of blood reveals a natural killer cell subset depletion in tuberculosis.

ABSTRACT:

Background

Tuberculosis (TB) continues to be a critical global health problem, which killed millions of lives each year. Certain circulating cell subsets are thought to differentially modulate the host immune response towards Mycobacterium tuberculosis (Mtb) infection, but the nature and function of these subsets is unclear.

Methods

Peripheral blood mononuclear cells (PBMC) were isolated from healthy controls (HC), latent tuberculosis infection (LTBI) and active tuberculosis (TB) and then subjected to single-cell RNA sequencing (scRNA-seq) using 10 × Genomics platform. Unsupervised clustering of the cells based on the gene expression profiles using the Seurat package and passed to tSNE for clustering visualization. Flow cytometry was used to validate the subsets identified b

SUBMITTER: Cai Y 

PROVIDER: S-EPMC7047188 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature