High-Throughput MICA/B Genotyping of Over Two Million Samples: Workflow and Allele Frequencies.

ABSTRACT: MICA and MICB are ligands of the NKG2D receptor and thereby influence NK and T cell activity. MICA/B gene polymorphisms, expression levels and the amount of soluble MICA/B in the serum have been linked to autoimmune diseases, infections, and cancer. In hematopoietic stem cell transplantation, MICA matching between donor and patient has been correlated with reduced acute and chronic graft-vs.-host disease and improved survival. Hence, we developed an extremely cost-efficient high-throughput workflow for genotyping MICA/B for newly registered potential stem cell donors. Since mid-2017, we have genotyped over two million samples using NGS amplicon sequencing for MICA/B exons 2-5. In donors of German origin, MICA*008 is the most common MICA alle

SUBMITTER: Klussmeier A 

PROVIDER: S-EPMC7047279 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature