Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins.

ABSTRACT: Green fluorescent proteins (GFPs) have become indispensable imaging and optogenetic tools. Their absorption and emission properties can be optimized for specific applications. Currently, no unified framework exists to comprehensively describe these photophysical properties, namely the absorption maxima, emission maxima, Stokes shifts, vibronic progressions, extinction coefficients, Stark tuning rates, and spontaneous emission rates, especially one that includes the effects of the protein environment. In this work, we study the correlations among these properties from systematically tuned GFP environmental mutants and chromophore variants. Correlation plots reveal monotonic trends, suggesting that all these properties are governed by one underlying factor dependent on the chromophore's envi

SUBMITTER: Lin CY 

PROVIDER: S-EPMC7047497 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature