Porous bio-click microgel scaffolds control hMSC interactions and promote their secretory properties.

ABSTRACT: Human mesenchymal stem/stromal cells (hMSCs) are known to secrete numerous cytokines that signal to endogenous cells and aid in tissue regeneration. However, the role that biomaterial scaffolds can play in controlling hMSC secretory properties has been less explored. Here, microgels were co-assembled with hMSCs using three different microgel populations, with large (190 ± 100 μm), medium (110 ± 60 μm), and small (13 ± 6 μm) diameters, to create distinct porous environments that influenced hMSC clustering. Cells embedded in large diameter microgel networks resided in large clusters (~40 cells), compared to small clusters (~6 cells) observed in networks using medium diameter microgels and primarily single cells in small diameter microgel networks. Using a cytokine microarray, an overall incr

SUBMITTER: Caldwell AS 

PROVIDER: S-EPMC7047645 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature