Parallel multi-parameter study of PEI-functionalized gold nanoparticle synthesis for bio-medical applications: part 1-a critical assessment of methodology, properties, and stability.

ABSTRACT: Cationic polyethyleneimine (PEI)-conjugated gold nanoparticles (AuNPs) that are chemically and physically stable under physiological conditions are an ideal candidate for certain bio-medical applications, in particular DNA transfection. However, the issue remains in reproducibly generating uniform stable species, which can cause the inadequate characterization of the resulting product under relevant conditions and timepoints. The principal objective of the present study was to develop an optimized and reproducible synthetic route for preparing stable PEI-conjugated AuNPs (Au-PEIs). To achieve this objective, a parallel multi-parametric approach involving a total of 96 reaction studies evaluated the importance of 6 key factors: PEI molar mass, PEI structure, molar ratio of PEI/Au, concentra

SUBMITTER: Cho TJ 

PROVIDER: S-EPMC7047743 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature