Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice.

ABSTRACT: In vivo electrophysiology is the gold standard technique used to investigate sub-second neural dynamics in freely behaving animals. However, monitoring cell-type-specific population activity is not a trivial task. Over the last decade, fiber photometry based on genetically encoded calcium indicators (GECIs) has been widely adopted as a versatile tool to monitor cell-type-specific population activity in vivo. However, this approach suffers from low temporal resolution. Here, we combine these two approaches to monitor both sub-second field potentials and cell-type-specific population activity in freely behaving mice. By developing an economical custom-made system and constructing a hybrid implant of an electrode and a fiber optic cannula, we simultaneously monitor artifact-free

SUBMITTER: Patel AA 

PROVIDER: S-EPMC7047771 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature