The detection of trans gene fragments of hEPO in gene doping model mice by Taqman qPCR assay.

ABSTRACT:

Background

With the rapid progress of genetic engineering and gene therapy methods, the World Anti-Doping Agency has raised concerns regarding gene doping, which is prohibited in sports. However, there is no standard method available for detecting transgenes delivered by injection of naked plasmids. Here, we developed a detection method for detecting transgenes delivered by injection of naked plasmids in a mouse model that mimics gene doping.

Methods

Whole blood from the tail tip and one piece of stool were used as pre-samples of injection. Next, a plasmid vector containing the human erythropoietin (hEPO) gene was injected into mice through intravenous (IV), intraperitoneal (IP), or local muscular (IM) injection. At 1, 2, 3, 6, 12, 24, and 48 h after injection, approximately

SUBMITTER: Aoki K 

PROVIDER: S-EPMC7047860 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature