Metabolic Engineering of Bacillus licheniformis for Production of Acetoin.

ABSTRACT: Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productivity. In this study, B. licheniformis MW3 was metabolic engineered for acetoin production. After deleting two 2,3-butanediol dehydrogenases encoding genes budC and gdh, an engineered strain B. licheniformis MW3 (?budC?gdh) was constructed. Using fed-batch fermentation of B. licheniformis MW3 (?budC?gdh), 64.2 g/L acetoin was produced at a productivity of 2.378 g/[L h] and a yield of 0.412 g/g from 156 g/L glucose in 27 h. The fermentation process exhibited rather high productivity and yield of acetoin, indicating that B. licheniformis MW3 (?budC?gdh) might be a promising acetoin producer.

SUBMITTER: Lu C 

PROVIDER: S-EPMC7047894 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature