The mismatch repair system (mutS and mutL) in Acinetobacter baylyi ADP1.

ABSTRACT:

Background

Acinetobacter baylyi ADP1 is an ideal bacterial strain for high-throughput genetic analysis as the bacterium is naturally transformable. Thus, ADP1 can be used to investigate DNA mismatch repair, a mechanism for repairing mismatched bases. We used the mutS deletion mutant (XH439) and mutL deletion mutant (XH440), and constructed a mutS mutL double deletion mutant (XH441) to investigate the role of the mismatch repair system in A. baylyi.

Results

We determined the survival rates after UV irradiation and measured the mutation frequencies, rates and spectra of wild-type ADP1 and mutSL mutant via rifampin resistance assay (Rif^R assay) and experimental evolution. In addition, transformation efficiencies of genomic DNA in ADP1 and its three mutants were deter

SUBMITTER: Zhou H 

PROVIDER: S-EPMC7048072 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature