Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis.

ABSTRACT: Oncogenic activation of RAS is associated with the acquisition of a unique set of metabolic dependencies that contribute to tumour cell fitness. Cells that express oncogenic RAS are able to internalize and degrade extracellular protein via a fluid-phase uptake mechanism termed macropinocytosis¹. There is increasing recognition of the role of this RAS-dependent process in the generation of free amino acids that can be used to support tumour cell growth under nutrient-limiting conditions². However, little is known about the molecular steps that mediate the induction of macropinocytosis by oncogenic RAS. Here we identify vacuolar ATPase (V-ATPase) as an essential regulator of RAS-induced macropinocytosis. Oncogenic RAS promotes the translocation of V-ATPase from intracel

SUBMITTER: Ramirez C 

PROVIDER: S-EPMC7048194 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature