S100B suppression alters polarization of infiltrating myeloid-derived cells in gliomas and inhibits tumor growth.

ABSTRACT: S100B, a member of the multigene family of Ca²⁺-binding proteins, is overexpressed by most malignant gliomas but its biological role in gliomagenesis is unclear. Recently, we demonstrated that low concentrations of S100B attenuated microglia activation through the induction of STAT3. Furthermore, S100B downregulation in a murine glioma model inhibited macrophage trafficking and tumor growth. Based on these observations, we hypothesized that S100B inhibitors may have antiglioma properties through modulation of tumor microenvironment. To discover novel S100B inhibitors, we developed a high-throughput screening cell-based S100B promoter-driven luciferase reporter assay. Initial screening of 768 compounds in the NIH library identified 36 hits with >85% S100B inhibitory activity. Dul

SUBMITTER: Gao H 

PROVIDER: S-EPMC7048242 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature