Membrane stabilizer Poloxamer 188 improves yield of primary isolated rat cardiomyocytes without impairing function.

ABSTRACT: Intact cardiomyocytes are used to investigate cardiac contractility and evaluate the efficacy of new therapeutic compounds. Primary enzymatic isolation of adult rodent cardiomyocytes has limitations, including low cardiomyocyte survival, which is likely due to ischemic conditions and/or membrane damage. The addition of Poloxamer 188 (P188) has been used to reduce ischemia- and membrane-related damage in ischemia-reperfusion and muscular dystrophy studies. P188 stabilizes membranes, reducing cell death. Cardiomyocytes were isolated from rats, under three conditions: (1) using standard isolation solutions, (2) with P188 added during cannulation (ischemic event), and (3) with P188 added during cannulation, enzymatic digestion, and trituration. Cell survival was assessed by quantifying the num

SUBMITTER: Czeiszperger TL 

PROVIDER: S-EPMC7048379 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature