Endometriotic cell culture contamination and authenticity: a source of bias in in vitro research?

ABSTRACT:

Study question

Are the primary cell cultures and cell lines used in endometriosis research of sufficient quality?

Summary answer

Primary cells used in endometriosis research lack purity and phenotypic characterisation, and cell lines are not genotypically authenticated.

What is known already

The poor reproducibility of in vitro research and the lack of authenticity of the cell lines used represent reasons of concern in the field of reproductive biology and endometriosis research.

Study design, size, duration

In the present study, past in vitro research in the field of endometriosis was systematically reviewed to determine whether the appropriate quality controls were considered. In addition, we explored the performance of Paired Box 2 (Pax2) as an endometrium s

SUBMITTER: Romano A 

PROVIDER: S-EPMC7048714 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature