Antibody transcytosis across brain endothelial-like cells occurs nonspecifically and independent of FcRn.

ABSTRACT: The blood-brain barrier (BBB) hinders the brain delivery of therapeutic immunoglobulin γ (IgG) antibodies. Evidence suggests that IgG-specific processing occurs within the endothelium of the BBB, but any influence on transcytosis remains unclear. Here, involvement of the neonatal Fc receptor (FcRn), which mediates IgG recycling and transcytosis in peripheral endothelium, was investigated by evaluating the transcytosis of IgGs with native or reduced FcRn engagement across human induced pluripotent stem cell-derived brain endothelial-like cells. Despite differential trafficking, the permeability of all tested IgGs were comparable and remained constant irrespective of concentration or competition with excess IgG, suggesting IgG transcytosis occurs nonspecifically and originates from fluid-pha

SUBMITTER: Ruano-Salguero JS 

PROVIDER: S-EPMC7048754 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature