Autoantigens in the trabecular meshwork and glaucoma-specific alterations in the natural autoantibody repertoire.

ABSTRACT:

Objectives

Primary open-angle glaucoma (POAG) is a neurodegenerative disorder leading to a gradual vision loss caused by progressive damage to the optic nerve. Immunological processes are proposed to be involved in POAG pathogenesis. Altered serological autoantibody levels have been frequently reported, but complete analyses of the natural autoantibodies with respect to disease-related alterations are scarce. Here, we provide an explorative analysis of pathways and biological processes that may involve naturally immunogenic proteins and highlight POAG-specific alterations.

Methods

Mass spectrometry-based antibody-mediated identification of autoantigens (MS-AMIDA) was carried out in healthy and glaucomatous trabecular meshwork (TM) cell lines, using antibody pools purified fr

SUBMITTER: Beutgen VM 

PROVIDER: S-EPMC7049230 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature