Metabolic characterisation of THP-1 macrophage polarisation using LC-MS-based metabolite profiling.

ABSTRACT:

Introduction

Macrophages constitute a heterogeneous population of functionally distinct cells involved in several physiological and pathological processes. They display remarkable plasticity by changing their phenotype and function in response to environmental cues representing a spectrum of different functional phenotypes. The so-called M1 and M2 macrophages are often considered as representative of pro- and anti-inflammatory ends of such spectrum. Metabolomics approach is a powerful tool providing important chemical information about the cellular phenotype of living systems, and the changes in their metabolic pathways in response to various perturbations.

Objectives

This study aimed to characterise M1 and M2 phenotypes in THP-1 macrophages in order to identify characterist

SUBMITTER: Abuawad A 

PROVIDER: S-EPMC7049298 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature