Identification and distribution of a single nucleotide polymorphism responsible for the catechin content in tea plants.

ABSTRACT: Catechins are the predominant products in tea plants and have essential functions for both plants and humans. Several genes encoding the enzymes regulating catechin biosynthesis have been identified, and the identification of single nucleotide polymorphisms (SNPs) resulting in nonsynonymous mutations within these genes can be used to establish a functional link to catechin content. Therefore, the transcriptomes of two parents and four filial offspring were sequenced using next-generation sequencing technology and aligned to the reference genome to enable SNP mining. Subsequently, 176 tea plant accessions were genotyped based on candidate SNPs using kompetitive allele-specific polymerase chain reaction (KASP). The catechin contents of these samples were characterized by high-performance liq

SUBMITTER: Jiang CK 

PROVIDER: S-EPMC7049304 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature