MicroRNA-based biomarkers for diagnosis of non-small cell lung cancer (NSCLC).

ABSTRACT:

Background

The development of biomarkers for the early detection of non-small cell lung cancer (NSCLC) is clinically important. We have developed miRNA biomarkers in sputum and plasma, respectively, for NSCLC. Herein, we evaluate whether integrated analysis of the miRNAs across the different types of specimens could improve the early detection of NSCLC.

Methods

Using reverse transcription PCR, we determined expressions of two miRNAs (miRs-31-5p and 210-3p) in sputum and three miRNAs (miRs-21-5p, 210-3p, and 486-5p) in plasma of a training cohort of 76 NSCLC patients and 72 cancer-free smokers. The results were validated in a testing cohort of 56 NSCLC patients and 55 cancer-free smokers.

Results

The panels of two sputum miRNAs and three plasma miRNAs had 65.8-75.0% se

SUBMITTER: Liao J 

PROVIDER: S-EPMC7049510 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature