Reducing the structure bias of RNA-Seq reveals a large number of non-annotated non-coding RNA.

ABSTRACT: The study of RNA expression is the fastest growing area of genomic research. However, despite the dramatic increase in the number of sequenced transcriptomes, we still do not have accurate estimates of the number and expression levels of non-coding RNA genes. Non-coding transcripts are often overlooked due to incomplete genome annotation. In this study, we use annotation-independent detection of RNA reads generated using a reverse transcriptase with low structure bias to identify non-coding RNA. Transcripts between 20 and 500 nucleotides were filtered and crosschecked with non-coding RNA annotations revealing 111 non-annotated non-coding RNAs expressed in different cell lines and tissues. Inspecting the sequence and structural features of these transcripts indicated that 60% of these trans

SUBMITTER: Boivin V 

PROVIDER: S-EPMC7049693 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature