Intracellular vesicle clusters are organelles that synthesize extracellular vesicle-associated cargo proteins in yeast.

ABSTRACT: Extracellular vesicles (EVs) play important roles in cell-cell communication. In budding yeast (Saccharomyces cerevisiae), EVs function as carriers to transport cargo proteins into the periplasm for storage during glucose starvation. However, intracellular organelles that synthesize these EV-associated cargo proteins have not been identified. Here, we investigated whether cytoplasmic organelles-called intracellular vesicle clusters (IVCs)-serve as sites for the synthesis of proteins targeted for secretion as EV-associated proteins. Using proteomics, we identified 377 IVC-associated proteins in yeast cells grown under steady-state low-glucose conditions, with the largest group being involved in protein translation. Isolated IVCs exhibited protein synthesis activities that required in

SUBMITTER: Winters CM 

PROVIDER: S-EPMC7049962 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature