Improved workflow for mass spectrometry-based metabolomics analysis of the heart.

ABSTRACT: MS-based metabolomics methods are powerful techniques to map the complex and interconnected metabolic pathways of the heart; however, normalization of metabolite abundance to sample input in heart tissues remains a technical challenge. Herein, we describe an improved GC-MS-based metabolomics workflow that uses insoluble protein-derived glutamate for the normalization of metabolites within each sample and includes normalization to protein-derived amino acids to reduce biological variation and detect small metabolic changes. Moreover, glycogen is measured within the metabolomics workflow. We applied this workflow to study heart metabolism by first comparing two different methods of heart removal: the Langendorff heart method (reverse aortic perfusion) and in situ freezing of mouse hea

SUBMITTER: Andres DA 

PROVIDER: S-EPMC7049973 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature