Nucleobase mutants of a bacterial preQ₁-II riboswitch that uncouple metabolite sensing from gene regulation.

ABSTRACT: Riboswitches are a class of nonprotein-coding RNAs that directly sense cellular metabolites to regulate gene expression. They are model systems for analyzing RNA-ligand interactions and are established targets for antibacterial agents. Many studies have analyzed the ligand-binding properties of riboswitches, but this work has outpaced our understanding of the underlying chemical pathways that govern riboswitch-controlled gene expression. To address this knowledge gap, we prepared 15 mutants of the preQ₁-II riboswitch-a structurally and biochemically well-characterized HL_out pseudoknot that recognizes the metabolite prequeuosine₁ (preQ₁). The mutants span the preQ₁-binding pocket through the adjoining Shine-Dalgarno sequence (SDS) and i

SUBMITTER: Dutta D 

PROVIDER: S-EPMC7049981 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature