MaXLinker: Proteome-wide Cross-link Identifications with High Specificity and Sensitivity.

ABSTRACT: Protein-protein interactions play a vital role in nearly all cellular functions. Hence, understanding their interaction patterns and three-dimensional structural conformations can provide crucial insights about various biological processes and underlying molecular mechanisms for many disease phenotypes. Cross-linking mass spectrometry (XL-MS) has the unique capability to detect protein-protein interactions at a large scale along with spatial constraints between interaction partners. The inception of MS-cleavable cross-linkers enabled the MS2-MS3 XL-MS acquisition strategy that provides cross-link information from both MS2 and MS3 level. However, the current cross-link search algorithm available for MS2-MS3 strategy follows a "MS2-centric" approach and suffers from a high rate of mis-identi

SUBMITTER: Yugandhar K 

PROVIDER: S-EPMC7050104 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature