Concentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation.

ABSTRACT: The use of protein biomarkers as surrogates for clinical endpoints requires extensive multilevel validation including development of robust and sensitive assays for precise measurement of protein concentration. Multiple reaction monitoring (MRM) is a well-established mass-spectrometric method that can be used for reproducible protein-concentration measurements in biological specimens collected via microsampling. The dried blood spot (DBS) microsampling technique can be performed non-invasively without the expertise of a phlebotomist, and can enhance analyte stability which facilitate the application of this technique in retrospective studies while providing lower storage and shipping costs, because cold-chain logistics can be eliminated. Thus, precise, sensitive, and multiplexed methods fo

SUBMITTER: Eshghi A 

PROVIDER: S-EPMC7050112 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature