Examining the Effect of Hypertonic Saline Administered for Reduction of Intracranial Hypertension on Coagulation.

ABSTRACT:

Background

Hypertonic saline (23.4%, HTS) bolus administration is common practice for refractory intracranial hypertension, but its effects on coagulation are unknown. We hypothesize that 23.4% HTS in whole blood results in progressive impairment of coagulation in vitro and in vivo in a murine model of traumatic brain injury (TBI).

Study design

For the in vitro study, whole blood was collected from 10 healthy volunteers, and citrated native thrombelastography was performed with normal saline (0.9%, NS) and 23.4% HTS in serial dilutions (2.5%, 5%, and 10%). For the in vivo experiment, we assessed the effects of 23.4% HTS bolus vs NS on serial thrombelastography and tail-bleeding times in a TBI murine model (n = 10 rats with TBI and 10 controls).

Results

For the in vitr

SUBMITTER: Coleman JR 

PROVIDER: S-EPMC7050279 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature