Current outcomes when optimizing 'standard' sample preparation for single-particle cryo-EM.

ABSTRACT: Although high-resolution single-particle cryo-electron microscopy (cryo-EM) is now producing a rapid stream of breakthroughs in structural biology, it nevertheless remains the case that the preparation of suitable frozen-hydrated samples on electron microscopy grids is often quite challenging. Purified samples that are intact and structurally homogeneous - while still in the test tube - may not necessarily survive the standard methods of making extremely thin, aqueous films on grids. As a result, it is often necessary to try a variety of experimental conditions before finally finding an approach that is optimal for the specimen at hand. Here, we summarize some of our collective experiences to date in optimizing sample preparation, in the hope that doing so will be useful to others, especia

SUBMITTER: Carragher B 

PROVIDER: S-EPMC7050573 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature