Nutrition assessment of vitamin A and vitamin D in northeast Chinese population based-on SPE/UPLC/PDA.

ABSTRACT:

Background

The aims of the current study were to assess the nutritional status of 25OHD3 and retinol in a northern Chinese population using our established reliable method for the simultaneous determination of serum 25OHD3 and retinol.

Method

We established a reliable method for the simultaneous determination of 25OHD3 and retinol using SPE and UPLC/PDA; measured the serum levels of 25OHD3 and retinol in elementary school students, middle school students, and adults (n = 1181) in northern China; and assessed their nutritional status.

Results

Our method had good precision, detection limit, and linear quantitative range and could process 100 samples within 12 h. The average levels of 25OHD3 and retinol were 16.1 ± 6.7 ng/ml and 328.1 ± 117.1 ng/ml, respectively,

SUBMITTER: Wang M 

PROVIDER: S-EPMC7050760 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature