Aberration correction for improving the image quality in STED microscopy using the genetic algorithm.

ABSTRACT: With a purely optical modulation of fluorescent behaviors, stimulated emission depletion (STED) microscopy allows for far-field imaging with a diffraction-unlimited resolution in theory. The performance of STED microscopy is affected by many factors, of which aberrations induced by the optical system and biological samples can distort the wave front of the depletion beam at the focal plane to greatly deteriorate the spatial resolution and the image contrast. Therefore, aberration correction is imperative for STED imaging, especially for imaging thick specimens. Here, we present a wave front compensation approach based on the genetic algorithm (GA) to restore the distorted laser wave front for improving the quality of STED images. After performing aberration correction on two types of zebra

SUBMITTER: Wang L 

PROVIDER: S-EPMC7051000 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature