Micro-respirometry of whole cells and isolated mitochondria.

ABSTRACT: Oxygen consumption is a key metric of metabolism in aerobic organisms. Current respirometric methods led to seminal discoveries despite limitations such as high sample demand, exchange with atmospheric O₂, and cumulative titration protocols leading to limited choice of useable tissue, complex data interpretation, and restricted experimental design. We developed a sensitive and customizable method of measuring O₂ consumption rates by a variety of biological samples in microliter volumes without interference from the aerobic environment. We demonstrate that O₂ permeability of the photopolymer, VeroClear, is comparable to that of polyetheretherketone (0.125 vs. 0.143 barrer, respectively) providing an efficient barrier to oxygen ingress. Optical transpa

SUBMITTER: Levitsky Y 

PROVIDER: S-EPMC7051014 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature