Efficient immortalization of human dental pulp stem cells with expression of cell cycle regulators with the intact chromosomal condition.

ABSTRACT: Clinical studies have recently demonstrated that autologous transplantation of mobilized dental pulp stem cells is a safe and efficacious potential therapy for pulp regeneration. However, some limitations need to be addressed, such as the high cost of the safety and quality control tests for isolated individual dental pulp cell products before transplantation. Therefore, more efficient in vitro culturing of human dental pulp stem cells might be useful for providing low cost and high reliability testing for pulp regeneration therapy. In this study, we established a novel immortalized dental pulp stem cell line by co-expressing a mutant cyclin-dependent kinase 4 (CDK4R24C), Cyclin D1, and telomerase reverse transcriptase (TERT). The established cell line maintained its original diploid chrom

SUBMITTER: Orimoto A 

PROVIDER: S-EPMC7051082 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature