Variability in protein cargo detection in technical and biological replicates of exosome-enriched extracellular vesicles.

ABSTRACT: Exosomes are extracellular vesicles (EVs) of ~20-200 nm diameter that shuttle DNAs, RNAs, proteins and other biomolecules between cells. The large number of biomolecules present in exosomes demands the frequent use of high-throughput analysis. This, in turn, requires technical replicates (TRs), and biological replicates (BRs) to produce accurate results. As the number and abundance of identified biomolecules varies between replicates (Rs), establishing the replicate variability predicted for the event under study is essential in determining the number of Rs required. Although there have been few reports of replicate variability in high throughput biological data, none of them focused on exosomes. Herein, we determined the replicate variability in protein profiles found in exosomes released

SUBMITTER: Tiruvayipati S 

PROVIDER: S-EPMC7051218 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature