Feasibility of the Bag-Mediated Filtration System for Environmental Surveillance of Poliovirus in Kenya.

ABSTRACT: The bag-mediated filtration system (BMFS) was developed to facilitate poliovirus (PV) environmental surveillance, a supplement to acute flaccid paralysis surveillance in PV eradication efforts. From April to September 2015, environmental samples were collected from four sites in Nairobi, Kenya, and processed using two collection/concentration methodologies: BMFS (> 3 L filtered) and grab sample (1 L collected; 0.5 L concentrated) with two-phase separation. BMFS and two-phase samples were analyzed for PV by the standard World Health Organization poliovirus isolation algorithm followed by intratypic differentiation. BMFS samples were also analyzed by a cell culture independent real-time reverse transcription polymerase chain reaction (rRT-PCR) and an alternative cell culture method (integrat

SUBMITTER: Zhou NA 

PROVIDER: S-EPMC7052051 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature