ACPAs promote IL-1β production in rheumatoid arthritis by activating the NLRP3 inflammasome.

ABSTRACT:

Objectives

Anti-citrullinated protein antibodies (ACPAs) are a group of autoantibodies targeted against citrullinated proteins/peptides and are informative rheumatoid arthritis (RA) biomarkers. ACPAs also play a crucial role in RA pathogenesis, and their underlying mechanism merits investigation.

Methods

Immunohistochemical (IHC) assays were carried out to determine IL-1β levels in ACPA⁺ and ACPA^- RA patients. PBMC-derived monocytes were differentiated into macrophages before stimulation with ACPAs purified from RA patients. The localization and interaction of molecules were analyzed by confocal microscopy, co-IP, and surface plasmon resonance.

Results

In our study, we found that IL-1β levels were elevated in ACPA⁺ RA patients and that

SUBMITTER: Dong X 

PROVIDER: S-EPMC7052171 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature