Transcriptional profiling of lung cell populations in idiopathic pulmonary arterial hypertension.

ABSTRACT: Despite recent improvements in management of idiopathic pulmonary arterial hypertension, mortality remains high. Understanding the alterations in the transcriptome-phenotype of the key lung cells involved could provide insight into the drivers of pathogenesis. In this study, we examined differential gene expression of cell types implicated in idiopathic pulmonary arterial hypertension from lung explants of patients with idiopathic pulmonary arterial hypertension compared to control lungs. After tissue digestion, we analyzed all cells from three idiopathic pulmonary arterial hypertension and six control lungs using droplet-based single cell RNA-sequencing. After dimensional reduction by t-stochastic neighbor embedding, we compared the transcriptomes of endothelial cells, pericyte/smooth mus

SUBMITTER: Saygin D 

PROVIDER: S-EPMC7052475 | biostudies-literature | 2020 Jan-Mar

REPOSITORIES: biostudies-literature