Roles of LRRC26 as an auxiliary γ1-subunit of large-conductance Ca²⁺-activated K⁺ channels in bronchial smooth muscle cells.

ABSTRACT: In visceral smooth muscle cells (SMCs), the large-conductance Ca²⁺-activated K⁺ (BK) channel is one of the key elements underlying a negative feedback mechanism that is essential for the regulation of intracellular Ca²⁺ concentration. Although leucine-rich repeat-containing (LRRC) proteins have been identified as novel auxiliary γ-subunits of the BK channel (BKγ) in several cell types, its physiological roles in SMCs are unclear. The BKγ expression patterns in selected SM tissues were examined using real-time PCR analyses and Western blotting. The functional contribution of BKγ1 to BK channel activity was examined by whole cell patch-clamp in SMCs and heterologous expression systems. BKγ1 expression in mouse bronchial SMCs (mBSMCs) was higher than in other

SUBMITTER: Noda S 

PROVIDER: S-EPMC7052682 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature