Identification of differentially expressed miRNAs and mRNAs in synovial of osteoarthritis via RNA-sequencing.

ABSTRACT:

Background

Osteoarthritis (OA) is the most common form of arthritis and a leading cause of disability. This study attempted to investigate the key mRNAs and miRNAs related to OA.

Patients and methods

From April 17th, 2018 to May 17th, 2018, five patients with OA and three normal controls were enrolled in this present study. To identify the differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs) between patients with OA and normal controls, RNA-sequencing was performed. Then, DEmiRNA-target DEmRNAs analysis and functional annotation of DEmiRNA-target DEmRNAs were performed. To validate the RNA-sequencing results, quantitative real time-PCR (RT-PCR) and western blot analysis were performed as well.

Results

A total of 1068 DEmRNAs, 21 DEmiRNAs and 395 DEmiRNA-DEmR

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC7053084 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature